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Variably improved microbial source tracking with digital droplet PCR
(Pergamon-Elsevier Science Ltd, 2019-08-01)
This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal ...
A Sigmoidal Model for the Interpretation of Quantitative PCR (QPCR) Experiments
(Universidad Militar Nueva Granada, 2016)
A quantitative real-time PCR method for in planta monitoring of Phytophthora infestans growth
(Wiley Blackwell Publishing, Inc, 2010-12)
Aims: To establish a reliable and rapid protocol to simultaneously obtain high quality DNA from an infected host plant and the infecting pathogen. To develop an accurate and sensitive low-cost assay for the quantification ...
Transcriptional expression analysis of oestrogen receptor in ovary of Texel and Araucana sheep breeds by real time quantitative RT-PCRAnálisis de la expresión transcripcional del receptor de estrógeno en ovario de ovejas prepúberes de razas Texel y Criolla Araucana mediante RT-PCR cuantitativo en tiempo real
(Archivos de Medicina Veterinaria, 2015)
Bias in Estimations of DNA Content by Competitive Polymerase Chain Reaction
(Academic Press Inc Elsevier Science, 2000-12)
Competitive PCR is a highly sensitive method for specific DNA quantification. Despite the lack of studies related to the accuracy of the method it has been widely used. Here we present a simulation model for competitive ...
Detection of Salmonella spp. in water using magnetic capture hybridization combined with PCR or real-time PCR
(IWA Publishing, 2006-03)
The removal of target DNA by magnetic capture hybridization (MCH) from constituents inhibitory to amplification by polymerase chain reaction (PCR) was evaluated using Salmonella as the test pathogen. Hybrids were subjected ...
Development and validation of a human DNA quantification and sex determination approach based on real time PCR followed by high resolution melting analysis
(Elsevier Ireland Ltd, 2015-12)
DNA quantitation is one of the most crucial factors affecting the success and quality of DNA typing by PCR. The aim of this work was to develop a DNA quantification assay to be used in routine forensic casework. It should ...
Specific Detection of Bovine Coronavirus N Protein with TaqMan Probe qRT-PCR
(Universidade Federal do Rio Grande do Sul, 2019)